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Genesis Biotech Inc. is different from other biotech companies which only acts as an intermediate agent to place orders for customers; Genesis Biotech Inc. is also capable to provide a wide range of in-house biotech products and service as well as related technical supports and assistance.¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@
¡´Just provide peptide sequences: Genesis Biotech Inc. can produce all of antibody products via epitope prediction, antigen design and peptide synthesis.¡@¡@¡@¡@¡@¡@¡@¡´Just provide target genes: We are able to process gene recombination, gene expression, protein purification and antibody preparation.
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¡´ 1 rabbit 2.5 mg purified antigen ( > 99%
purity¡B1.0 mg / ml )
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2 rabbits 4.5 mg purified antigen ( > 99% purity¡B1.0 mg / ml )
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2 rats 1.0 mg purified antigen ( > 99% purity¡B1.0 mg / ml )
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3 rats 1.2 mg purified antigen ( > 99% purity¡B1.0 mg / ml )
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3 mice 1.2 mg purified antigen ( > 99% purity¡B1.0 mg / ml )
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¡´The best concentration of the antigen is larger than 1£gg/£gl. Please label the exact concentration.¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@
¡´Please dissolve the antigen in a nonpoisonous and bio-affordable buffer (such as PBS, Tris, Borate, Phosphate, etc
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If the client provide the antigen in SDS-PAGE, we suggest them prepare the antigen following the pre-process below. Or animals will die from being injected with large concentration of acrylamide gel antigen.¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@
¡´Concentrate the sample as possible as you can.
¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@
¡´Load the sample in one piece of acrylamide gel if possible.¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@
¡´Run mingled or std. Gels (1 mm or 1.5 mm thickness). Use a big flat comb with no
wells.¡@ ¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@
¡´Stain the gel with coomassie blue and then destain it. (Decrease the toxicity of acrylamid gel.)¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@
¡´Cut the clear and firm band out of the gel. Carefully cut along the edge of the
band.
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Of course, you just need to provide gene sequences or
gene names, our company engineer would design peptide sequence and
synthesize peptides what you need. Computer analysis and expert
selection of the most antigenic portion of your protein is included
with all custom antibody packages. |
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¡´Our company will pick up the antigen from you.¡@¡@¡@¡@¡@¡@¡@¡@¡@
¡´You can deliver the antigen to our company by a logistics company (eg. Takkyubin).¡@
¡´Overseas customers can send the antigen by FedEx. (Customers need to pay for the delivery fee.)
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1 rabbit 50 ml anti-serum
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2 rabbits 100 ml anti-serum
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2 rats 6.0 ml anti-serum
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3 rats 9.0 ml anti-serum
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3 mice 1.2 ml anti-serum |
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We will test the titer of the antibody by ELISA or
Western Blot, and assure it will be larger than 10 K. Before
immunizing animals, we will take blood samples to test the titer of
the antibody. The client can also ask for testing blood during
process. |
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We complete the projects within a short period of time.¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@
¡´We usually inform our customers about the progress by telephone or e-mail.¡@¡@¡@¡@
¡´We will inquire the feedback from our customers.¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@
¡´We will give discount when the customers reorder.
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¡´We will update information about our products and deliver to our
customers.
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Antibody purification will increase the reliability of your experiment, and decrease cross-reaction and non-specific reaction of
experiment.
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¡´A sale person from our company will deliver the frozen antibody.¡@¡@¡@¡@¡@¡@¡@¡@
¡´We will send by home delivery with a iced container at temperature of 4¢J to the customers in Taiwan.¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@
¡´For the overseas customers, we will ship the antibody by FedEX with a iced container at temperature of 4¢J. The customers need to pay for the postage.
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¡´Avoid repeating freeze/thaw cycles.
¡´Divide the antibody solution into small quantity.¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@
¡´For a short storage, keep the products at the temperature of 4¢J in a refrigerator.¡@
¡´For a long storage, keep the products at the temperature of -20¢Jor -80¢J in a freezer.
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